Shimae Nafarzadeh, Mina Motallebnejad, Anahita Ghorbani, Naghmeh Jamaatlou,
Volume 3, Issue 1 (3-2014)
Abstract
Introduction:Neurofibromatosis is a genetic disease characterized by multifocal benign tumors of peripheral nerves, called neurofibromas, and pigmented spots on the skin which inherited as autosomal-dominant. The most common form of the disease is neurofibromatosis type 1, also known as von Recklinghausen's disease of the skin. When an individual has small number of lesions in a limited region of the his body, it could be missed by the patient or not acknowledged by the clinicians as a form of neurofibromatosis.We present here, a case of an 18-year-old male with neurofibromatosis type 1who referred to Babol Dental School for a routine dental examination.
Ali Dehghani Nazhvani, Zohreh Jafari Ashkavandi, Nafiseh Shamloo, Zeinab Moniri,
Volume 3, Issue 2 (9-2014)
Abstract
Introduction: Diagnosis of neurofibroma usually is based on the specific morphology and arrangement of mesenchymal cells in routine Hematoxylin and Eosin (H&E) sections, and detection of mast cells supports the diagnosis. Sometimes definite diagnosis from other mesenchymal lesions may be difficult. The aim of the present study was to compare S100 expression and mast cells count (as Gold Standard) with routine histopathologic diagnosis.
Methods: In this cross-sectional analytical study, all cases of neurofibroma and compatible/ consistent with neurofibroma, that had been diagnosed in department of oral & maxillofacial pathology, school of dentistry, Shiraz, from 1986 to 2013, were enrolled. Immunohistochemistry was performed using S100 antibody and slides were stained by Giemsa. S100 labeling index, intensity and distribution as well as mast cells count were evaluated using light microscope.
Results: Mast cells were present in 97% of cases that 56.4 % showed 1-200 cells/10HPF. 82 % of cases were positive for S100 that 40.7% showed 2-30% labeling index and 70.4% had moderate intensity for S100 staining.
Conclusions: The comparison of routine histopathologic examination with gold standard method in Oral Pathology Department of Shiraz Dental School confirmed the routine histopathologic diagnosis in all cases, therefore no more evaluation may be required if a pathologist considers all routine diagnostic criteria.
Mahsa Dastpak, Shima Nafarzadeh, Soraya Khafri,
Volume 4, Issue 1 (3-2015)
Abstract
Introduction: Oral squamous cell carcinoma (OSCC) is one of the 10 most common malignant tumors and SCC accounts 94% of all oral malignancies. Mast cells are regarded as complex and multifunctional cells, playing a significant role in immunopathology . The aim of this study is to evaluate the number of mast cells in tissue sections of oral squamous cell carcinoma (OSCC) in comparison with normal mucosa.
Materials & Methods: Sixty paraffin-embedded specimens were obtained from the archives of the Department of Oral and Maxillofacial Pathology,dental school of Babol university of medical science (15 high grade,15 low grade and 30 Iritation Fibroma). Classification of OSCC cases was according to the BRODER`S malignancy grading system. Hematoxylin and Eosin-stained slides were re-evaluated before entering the samples in our study. Toluidine blue(1%) staining was used to identify Mast cells in samples . We used SPSS software version 18 and one way ANOVA test for analyzing data.
Results: The highest mast cell count was seen in normal tissue and it was higher in low grade OSCC in comparison with high grade, but the differences between groups weren’t statistically significant. The Mean count of mast cell between OSCC and normal oral mucosa was statistically significant different(p=0.019).We didn’t observe any statistically significant difference between Mast cell counts of control group and low grade OSCC . The same result was seen between high garde and low grade OSCC . The Mean mast cell count difference between male and female groups weren’t statistically significant. The Mean mast cell count difference between high grade OSCC and control group was significant (p<0.05).
Conclusion: According to the results, the average amount of mast cells decreased in OSCC in comparison with normal oral mucosa . It does not seem that mast cells play an important role in tumor progression, although further study is needed.
Hamideh Kadeh, Shirin Saravani, Babak Moradbeiki,
Volume 4, Issue 2 (9-2015)
Abstract
Introduction: The geographical distribution of head and neck cancer shows considerable variation and it is site dependent. The review of the literature shows few data concerning the head and neck cancer patterns in Iran. The purpose of this study was to represent epidemiological aspects of head and neck cancers in south east of Iran.
Materials &Methods: In this retrospective study, from a total of 924 diagnosed patients with cancer in the pathology department of dentistry school archives and two main hospitals in south east of Iran during 2006-2012, 287 cases of head and neck cancer were diagnosed histopathologically . Demographic data of patients such as age, gender, location and type of cancer (carcinoma, sarcoma, melanoma, metastatic, or primary) were obtained from patients’ records. Data were analyzed by SPSS statistical software (V.21) using the chi-square test.
Results: Squamous cell carcinoma (39.7%) and basal cell carcinoma (26.8%) were the most common malignancies in the head and neck region. The mean age of the patients was 56.1±12.8 years and the relationship between age groups and head and neck cancers was not statistically significant (P=0.085). Male to female ratio was 0.9 to 1. The relationship between gender and types of head and neck cancers was not statistically significant.(P=0.114)
Conclusion: In this study, prevalence of head and neck cancer in south east of Iran (Zahedan) was relatively high. However, comparison between findings of our study with other studies shows a relation consistency.
Ali Dodani, Sepideh Siadati, Jahanshah Salehinejad, Karimollah Hajian-Tilaki, Hamid Abbaszadeh,
Volume 5, Issue 2 (9-2016)
Abstract
Introduction: Differentiation of fibroblasts to myofibroblasts is an initial and very important event in tumor genesis. Myofibroblasts produce proteinases that stimulate invasion in cancers. Due to the more malignant potential of oral squamous cell carcinoma (OSCC) compared to cutaneous squamous cell carcinoma (CSCC) , the aim of the present study was to compare myofibroblasts between OSCC and CSCC to understand whether myofibroblasts can help more malignant potential of OSCC compared to CSCC or not.
Materials &Methods: This cross-sectional study included 40 cases of OSCC and CSCC and 20 cases of normal skin and normal oral mucosa. Then, 4-micron sections of paraffin-embedded tissue blocks of studied groups were stained immunohistochemically with α-SMA antibody. Mean percentage of myofibroblasts was calculated in invasive fronts of OSCCs with CSCCs and also in normal samples and staining intensity of cells for α-SMA marker and distribution pattern of myofibroblasts were determined.
Results: The differences of average percentage of myofibroblasts in OSCC and CSCC compared to normal groups were significant (Pvalue=0.007and Pvalue=0.003 respectively), but when we compared OSCCs and CSCCs, the difference was not significant. Also, there were no significant differences between OSCC and CSCC with regard to staining intensity and pattern.
Conclusion: Different biologic behavior of OSCC compared to CSCC doesn’t depend on myofibroblasts and other factors can be involved.
Mohammad Koochek Dezfuli, Maryam Seyedmajidi, Shabnam Sohanian, Hamed Hossein Kazemi, Khashayar Dehshiri,
Volume 6, Issue 1 (3-2017)
Abstract
Plexiform schwannoma is a type of schwannoma that can be occured at any age and any site of the oral cavity. In the gross and histopathological features, it shows plexiform or multinodular growth pattern. This variant of schwannoma is sometimes associated with neurofibromatosis type II or schwannomatosis. Histopathologically, plexiform schwannoma is composed of schwann cells that show Anthony A growth pattern. Mitosis are rare or absent.This study reports a case of a healthy 34 year old female with swelling in floor of her mouth; according to the clinical and histopathological findings, the diagnosis was Plexiform schwannoma.
Shirin Saravani, Hamideh Kadeh, Asma Abil Najafzade,
Volume 6, Issue 2 (9-2017)
Abstract
Introduction: Although recent studies show the association of eosinophils with different tumors including oral malignancies, the role of eosinophils in premalignant conditions of the oral cavity is not known precisely. Therefore, this study aimed to determine the number of eosinophils in premalignant and malignant conditions of the oral cavity and its role in progression to malignancy.
Materials &Methods: This cross-sectional study was done on 50 samples with oral squamous cell carcinoma (OSCC), 18 with verrucous carcinoma, 13 with erosive lichen planus, and 10 with leukoplakia with mild dysplasia after confirmation of histopathological diagnosis. The samples were stained with Sirius Red. The total numbers of eosinophils in 10 areas were counted at a magnification of 400x (eosinophil/10HPF). Data are analyzed with Kruskal-Wallis test, Mann-Whitney test, and Spearman’s correlation test.
Results: The mean number of eosinophils in OSCC, verrucous carcinoma, erosive lichen planus, and leukoplakia with mild dysplasia was 90.26, 59.72, 11.08 and 0.0 eosinophil/10HPF respectively. The mean number of eosinophils was significantly higher in malignant (OSCC and verrucous carcinoma) than premalignant (erosive lichen planus and leukoplakia with mild dysplasia) lesions of the oral cavity (P=0.0001). No significant correlation was found between the number of eosinophils with histopathologic grade, gender, and age in OSCC samples.
Conclusion: The greater number of eosinophils is seen in malignant than premalignant lesions of the oral cavity. It is recommended to consider the high number of eosinophils in premalignant lesions as an indicator for a more potential of preliminary oral lesions to undergo malignant transformation.
Azadeh Majidian, Maryam Seyedmajidi, Hamid Abbaszadeh, Majid Sharbatdaran, Sina Jafari, Hemmat Gholinia,
Volume 9, Issue 2 (9-2020)
Abstract
Introduction: Oral lichen planus (OLP) is an immunologically mediated mucocutaneous diseases. Langerhans cells (LCs) are antigen presenting cells resident within oral mucosa which, together with intraepithelial lymphocytes, play a role in mucosal defense. The aim of present study was to determine the immunohistochemical expression of LC in oral lichenoid reaction (OLR) and OLP compared with normal oral mucosa (NOM).
Materials & Methods: In this cross-sectional study, 61 oral mucosal specimens diagnosed histopathologically as OLP (n=31) and OLR (n=30) and 11 NOM were used to study the immunohistochemical expression of CD1a. The CD1a positive LCs in three regions (basal, suprabasal, and connective tissue) were counted in six randomly selected fields. Data were analyzed using the SPSS 24, T-test, ANOVA, LSD, Games-Howell tests. P<.05 was considered significant.
Results: CD1a expression in OLP and OLR was significantly higher compared to NOM (P<0.001). CD1a expression in OLP was also significantly higher than OLR (P=0.009). There was a statistically significant difference between the three groups in basal, suprabasal and connective tissue regions (P<0.001). The mean of CD1a positive cells in the basal, suprabasal and connective tissue regions was significantly higher in the OLP group than OLR and NOM. Also, in comparison between OLR and NOM, the OLR group had significantly more CD1a positive cells (P<0.05).
Conclusion: Increasing the number of CD1a in OLP and OLR compared with the NOM indicates the role of LC in the pathogenesis of these diseases.
Zahra Shokri, Maryam Seyedmajidi, Mahdi Pouramir, Ali Bijani, Raheleh Ahmadian, Mina Motallebnejad,
Volume 11, Issue 2 (9-2022)
Abstract
Introduction: Oral lichen planus (OLP) is a chronic mucocutaneous inflammatory disorder with an unknown etiology. Although a considerable body of evidence suggests that immunologic factors are involved in the etiology of OLP, the involvement of cytokines in the pathogenesis of the disease is not fully understood yet. The aim of the present study was to assess interleukin-6 (IL-6) levels as a proinflammatory cytokine in the saliva of OLP patients compared to healthy controls.
Materials & Methods: This case-control study was conducted on 30 OLP patients (12 males and 18 females) and 30 healthy control subjects, selected from individuals who were referred to the Department of Oral and Maxillofacial Diseases in Faculty of Dentistry, Babol University of Medical Sciences. Samples of unstimulated saliva were collected. Salivary IL-6 levels were measured using an ELISA kit and compared between OLP patients and healthy controls. The collected data were analyzed by Chi-square, independent t-test, and receiver operating characteristic (ROC) curve using SPSS 18 Sensitivity, specificity, positive predictive values (PPVs), and negative predictive values (NPVs) were calculated. A value of p<0.05 was considered significant.
Results: Mean salivary IL-6 values in OLP patients and healthy controls were 24.68±9.90 ng/L and 13.76±9.27 ng/L, respectively. The difference was statistically significant (P<0.001). The mean IL-6 values in reticular and erosive forms of OLP clinically were 24.35±9.26 ng/L and 24.91±10.64 ng/L, respectively. This difference was not statistically significant (P=0.87).
Conclusions: Higher levels of IL-6 in saliva of OLP patients compared with healthy controls support the role of IL-6 in the pathogenesis of the disease.
Maryam Seyedmajidi, Tahereh Momeni, Fatima Bijani,
Volume 12, Issue 2 (9-2023)
Abstract
Calcifying odontogenic cyst (COC) is an odontogenic cyst with accumulation of ghost cells that has central and peripheral subsets. Here, we present an 11-year-old male patient with an intraoral swelling on the left posterior mandibular ridge interfering with eruption of the second molar. Histopathologic examination revealed a cystic lesion lined by ameloblast-like epithelium in the basal layer and accumulating ghost cells in the upper layers. The final diagnosis was peripheral COC, and there was no recurrence after 6 months. This case has shown that histologic examination is required for every simple case.
Zahra Zolfaghari Saravi, Maryam Seyedmajidi, Ehsan Moudi, Fatima Bijani, Arezoo Javani,
Volume 13, Issue 1 (3-2024)
Abstract
Odontogenic kerato Cyst (OKC) is a developmental odontogenic cyst with specific histopathologic features and clinical behavior. Several investigators suggested that OKCs should be considered benign cystic neoplasms rather than cysts. OKCs are locally aggressive benign tumors with a high recurrence rate. Usually, multiple OKCs are observed in nevoid basal cell carcinoma syndrome (NBCCS) but about 5% of OKC have multiple cysts without an accompanying syndrome.
Mutation in the human homolog of the Drosophila segment polarity gene, “patched” ((PTCH) gene) is seen. Multiple recurrent OKCs without associated systemic findings are related to the partial expression of the gene. However, few cases of non-syndromic multiple keratocystic odontogenic tumors have been reported in the literature. This case report presents a rare case of an 11-year-old boy with multiple OKCs without any syndromic association. The patient underwent surgery for multiple and recurrent cysts in the jaws over 5 years. The patient was screened for syndromic diseases and no signs or symptoms of the syndrome were found.
Keywords: Odontogenic Cysts, Gorlin Syndrome, Neoplasms.
