Volume 7, Issue 1 ( March- 2018)
Caspian J Dent Res 2018, 7(1): 37-42 |
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Saeidi A, Hamidi M R, Davoodabadi A, Mahmoudi E, Khafri S, Nikouee Rad Z. Comparison of antibacterial effect of methanolic and hydro-alcoholic ziziphus spina-christi extract with 2.5% sodium hypochlorite on enterococcus faecalis: an in vitro study. Caspian J Dent Res 2018; 7 (1) :37-42
URL: http://cjdr.ir/article-1-229-en.html
URL: http://cjdr.ir/article-1-229-en.html
Akam Saeidi 
, Mahmoud Reza Hamidi , Abolfazl Davoodabadi , Elham Mahmoudi , Soraya Khafri , Zahra Nikouee Rad *
, Mahmoud Reza Hamidi , Abolfazl Davoodabadi , Elham Mahmoudi , Soraya Khafri , Zahra Nikouee Rad *
,Student Research Committee, Babol University of Medical Sciences, Babol, IR Iran. , nikouee96@yahoo.com
Abstract: (8572 Views)
Introduction: Root treatment will not be successful, without a proper root canal irrigation with less disadvantages and antibacterial effect. The aim of this study was to compare antimicrobial effect of cedar extract and 2.5%NaOCl on E. faecalis.
Materials & Methods: In disk diffusion test, a standard suspension of E. faecalis (ATCC 29212) was cultured on plate and different concentrations (0.05, 0.15, 0.25, 0.35, 0.45 g/ml) of methanolic or hydro-alcoholic extracts, 2.5% NaOCl and physiologic serum (as negative control) were infused on paper disks. The inhibition zone measured after 48h. In microdilution test, serial dilution of methanolic and hydro-alcoholic extracts, 2.5% NaOCl and physiologic serum in 1:2 proportion was performed in Brain Heart Infusion (BHI) culture medium. Then, standard suspension of E. faecalis was added to each well of micro plate. Data were analyzed using ANOVA.
Results: Hydro-alcoholic and methanolic extracts had antibacterial effect on E. faecalis. Inhibition zone of 2.5% NaOCl was significantly higher than that of other extracts (p<0.001). In microdilution test, E. faecalis bacterium was sensitive to both hydro-alcoholic and methanolic extracts but it was more sensitive to 2.5% NaOCl.
Conclusion: Totally, 2.5% NaOCl had the highest antibacterial effect on E.faecalis followed by hydro-alcoholic and methanolic extracts. NaOCl is an effective irrigant in root treatment until the studies like this can find a good alternative for it.
Materials & Methods: In disk diffusion test, a standard suspension of E. faecalis (ATCC 29212) was cultured on plate and different concentrations (0.05, 0.15, 0.25, 0.35, 0.45 g/ml) of methanolic or hydro-alcoholic extracts, 2.5% NaOCl and physiologic serum (as negative control) were infused on paper disks. The inhibition zone measured after 48h. In microdilution test, serial dilution of methanolic and hydro-alcoholic extracts, 2.5% NaOCl and physiologic serum in 1:2 proportion was performed in Brain Heart Infusion (BHI) culture medium. Then, standard suspension of E. faecalis was added to each well of micro plate. Data were analyzed using ANOVA.
Results: Hydro-alcoholic and methanolic extracts had antibacterial effect on E. faecalis. Inhibition zone of 2.5% NaOCl was significantly higher than that of other extracts (p<0.001). In microdilution test, E. faecalis bacterium was sensitive to both hydro-alcoholic and methanolic extracts but it was more sensitive to 2.5% NaOCl.
Conclusion: Totally, 2.5% NaOCl had the highest antibacterial effect on E.faecalis followed by hydro-alcoholic and methanolic extracts. NaOCl is an effective irrigant in root treatment until the studies like this can find a good alternative for it.
Type of Study: Research Paper |
Subject:
Endodontics
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