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:: Volume 6, Number 1 (March- 2017) ::
CJDR 2017, 6(1): 29-35 Back to browse issues page
The comparison of antibacterial effect of propolis , sodium hypochlorite 5.25%, and chlorhexidine 2% as intracanal irrigants against enterococcus faecalis: an ex vivo study
Maryam Zare Jahromi, Arezoo Tahmoorespoor, Nadia Hemmat, Elham Moghadasi Broujeni, Parisa Ranjbarian *
Assistant Professor ,Department of Endodontics, Faculty of Dentistry, Shahrekord University of Medical Sciences , Parian_1381@yahoo.com
Abstract:   (446 Views)

Introduction: Debridement of root canal using appropriately safe and effective irrigants is the key factor for long-term success. Purpose of this study was to compare the antibacterial effect of propolis with 5.25% sodium hypochlorite, and 2% chlorhexidine against enterococcus faecalis.

Materials &Methods: In this study, 36 single-canal roots were used. The crown was removed and instrumentation was prepared by step-back technique, then teeth were sterilized and contaminated with E. Faecalis, and divided into four groups with 9 cases: group1: Propolis, group2: 5.25% sodium hypochlorite, group3: 2% chlorhexidine and group4: controls. Irrigants were injected by a 27-gauge syringe and roots were incubated in 37°C for one week. Sampling was done and inoculated to tryptone soy broth media, after 24 hours the turbidity was measured. Samples were also cultured on agar plates, and colony-forming units were counted as CFU/ml. Data were analysed using the Mann-Whitney test.

Results: The difference between propolis with mean value of 246.77 colonies and chlorhexidine with mean value of zero colonies, was significant (P=.002). Similarly, the difference between chlorhexidine and sodium hypochlorite with mean value 203.55 of colonies was significant and they had significant difference in turbidity (P=.002), too. No significant difference was observed between propolis and sodium hypochlorite with regard to the induced colonies (P=0.781) and their turbidity (P=0.495).

Conclusion: It can be concluded that antibacterial activity of 2% chlorhexidine against E. faecalis is more obvious than propolis or 5.25% sodium hypochlorite. But antibacterial activity of propolis over 5.25% sodium hypochlorite or vice versa was not confirmed.

Keywords: Enterococcus faecalis, Propolis, Sodium hypochlorite, Chlorhexidine, Root canal therapy
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Type of Study: Original Article | Subject: Endodontics
* Corresponding Author Address: Shahrekord,Iran.
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DOI: 10.22088/acadpub.BUMS.6.1.29


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Zare Jahromi M, Tahmoorespoor A, Hemmat N, Moghadasi Broujeni E, Ranjbarian P. The comparison of antibacterial effect of propolis , sodium hypochlorite 5.25%, and chlorhexidine 2% as intracanal irrigants against enterococcus faecalis: an ex vivo study. CJDR. 2017; 6 (1) :29-35
URL: http://cjdr.ir/article-1-197-en.html
Volume 6, Number 1 (March- 2017) Back to browse issues page
مجله تحقیقات دندانپزشکی کاسپین Caspian Journal of Dental Research
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